Molecular Diagnostic Training

Practical training in PCR and molecular diagnostic workflows, from specimen preparation through sequence analysis and interpretation.

Practical coverage

• DNA and RNA extraction and nucleic acid quality assessment
• Primer handling, PCR reagent preparation and conventional PCR
• Gradient PCR, optimisation, contamination control and troubleshooting
• Agarose gel electrophoresis and gel documentation
• DNA sequencing workflows, DNA barcoding and sequence analysis

Advanced exposure

• STR analysis concepts and fragment-analysis workflows
• Species identification and parentage-testing principles
• Molecular veterinary diagnostics

Instrumentation exposure

• Bio-Rad Gradient PCR
• UV spectrophotometer and NanoDrop
• Gel documentation and electrophoresis systems
• Centrifuges and supporting molecular laboratory equipment

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